Los Angeles, which has more storefront medical marijuana shops than any other US city, has already shut down more than 100 dispensaries, according to City Attorney Mike Feuer.
Reuters reports that the remaining dispensaries will be subject to harsher tax regulations.
These new measures against the pot shops are part of proposition D, which passed with 63 percent of Los Angeles voters last May. The proposition caps the number of dispensaries in the city at 135.
According to the LA Times, Feuer said he has already hired two new attorneys to prosecute cases that fall exclusively under proposition D.
Officials are also focusing on deterring landlords renting space for marijuana shops, providing them with pamphlets that warn of the steep punishment for breaking the law.
LAPD chief Charlie Beck announced that new training would provide neighborhood patrol officers with the information to detect new and existing illegal dispensaries.
As there is no current permit process, there's no set number on how many of the dispensaries currently exist Los Angeles, but most estimate it currently exceeds 700.
The city's financial administration is still providing prospective dispensary owners with registration certificates, which don't necessarily function as a legal permit but give the impression of one. Without coordination between the City Attorney's office and the financial office, some argue this creates confusion.